Being rich in lignocellulosic content, these can be used in production of many value added products such as enzymes, paper, paperboard, etc.
with simultaneous reduction in the environmental load.
Cellulase production also requires the development of a suitable bioprocess and the medium for fermentation constituting a cheap substrate such as wheat bran which is a low-cost agro-industrial residue.
Since the enzyme production from microbial source is a multivariable controlled process, it becomes essential to optimize the key factors i.e.
In order to achieve high titer of cellulase, a central composite design (CCD) was constructed and performed for optimization of SSF with five process variables at five coded levels.
A 2 full factorial design was constructed leading to a set of 50 experiments that were performed in triplicates.Hence, response surface methodology (RSM) was applied as it is a useful statistical technique for analyzing the effect of several independent variables.A central composite design (CCD) was constructed to evaluate the interactive and mutual impact of the key variables on cellulase production with an objective to obtain high yields of cellulase in a cost effective and eco-friendly bioprocess.Bhardwaj, Jitender Sharma (2018) Lignocellulosic Waste as a Sole Substrate for Production of Crude Cellulase from Bacillus subtilis PJK6 Under Solid State Fermentation Using Statistical Approach. Agro residues are abundantly produced as byproducts during crop production and cause major environmental issues for their disposal and management.Stubble burning has become prominent due to factors such as increased mechanization, reduced livestock number, long duration for composting and unavailability of alternate economically viable solutions .Typical examples of such sites from where the samples were collected include sludge from biogas plant, cow dung, degraded litter, degrading lignocellulosic biomass, soil from agricultural fields, sludge from paper mill, deteriorating sugarcane in soil, kitchen garden soil, decaying wood etc.All the samples collected were divided in two parts and one part of each was heat treated by keeping at 60 The microorganisms producing cellulase were isolated by Enrichment technique using 1% CMC (Carboxy Methyl Cellulose) based on the hypothesis that if the microorganisms are provided with cellulose as a sole carbon source in the medium for their growth, then only those who can produce cellulase enzyme to utilize cellulose for their growth and the metabolic needs could survive.This presents the requirement of economic production of cellulase to make it more feasible and cost effective for industrial use.Microorganisms have widely been exploited as a major source for production of a diverse range of industrial enzymes.Moreover, they are known to utilize a wide range of substrates that include solid organic waste residues from agriculture, forest, mills etc.to provide concurrent benefit of waste management with economic production of enzymes.