If using a cork borer, each group (at least) should use a bores of the same diameter.This causes a reduction in the tension of the cell wall and brings about the contraction of protoplasm due to the continuous loss of water.The protoplasm becomes rounded in shape due to contraction and such a cell is said to beplasmolysed and the phenomenon is referred to as plasmolysis.Measure out water using the volumes shown in column 5. The advantage of using blackcurrant squash is that students can see at a glance which is the most concentrated solution.(Columns 2 and 3 show the volumes needed to make 10 cm3 of solution, which is multiplied to get larger volumes in columns 4 and 5.) c Collect some potato chips or a potato to cut up. e Weigh the chips using a balance; record masses in an appropriately designed table. This means they are less likely to get the concentrations confused in the course of the procedure.This potato is then placed in a beaker containing coloured water for some time.When a plant cell is placed in hypertonic solution, the process of exosmosis starts and water from the cell sap diffuses out into the solution of external medium.Cut or trim your chips, if necessary to fit into your small beaker/ boiling tube. However, for A-level studies you would need to convert the concentrations into molar equivalents, and process the data on graphs showing standard deviation bars in order to assess the variation in repeats.At A-level, graph points should be joined with ruled lines and show error bars.Make qualitative observations or calculate the percentage change in mass.Each working group (or individual students) could carry out the complete protocol.